Journal: Biochemistry

Article Title: STABILITY AND STOICHIOMETRY OF BILAYER PHOSPHOLIPID-CHOLESTEROL COMPLEXES: RELATIONSHIP TO CELLULAR STEROL DISTRIBUTION AND HOMEOSTASIS &

doi: 10.1021/bi400862q

Figure Lengend Snippet: Examples of time courses of oxidation of LUV cholesterol. LUV phospholipid (PL), cholesterol mole fraction (CMF) and cholesterol oxidase (CO) were varied as follows. POPC (PL = 0.62 mM, CMF = 0.43, CO = 1 U/ml), (○); DOPC (PL = 0.71 mM, CMF = 0.26, CO = 0.04 U/ml), (●); PSM (PL = 0.25 mM, CMF = 0.61, CO = 10 U/ml), (▽); DMPC (PL = 0.40 mM, CMF = 0.52, CO = 2 U/ml), (□); Minus LUVs (CO = 2 U/ml), (◇).

Article Snippet: The Amplex Red Cholesterol Assay kit was from Life Technologies.

Techniques:

Journal: Biochemistry

Article Title: STABILITY AND STOICHIOMETRY OF BILAYER PHOSPHOLIPID-CHOLESTEROL COMPLEXES: RELATIONSHIP TO CELLULAR STEROL DISTRIBUTION AND HOMEOSTASIS &

doi: 10.1021/bi400862q

Figure Lengend Snippet: Dependence of the relative accessibility (RA) of cholesterol to cholesterol oxidase on membrane cholesterol. CMF, cholesterol mole fraction. [Data points are in arbitrary units calculated according to eq. (6) in Appendix 1]. Solid lines, best fits using the values given in Table 1, columns a-d; however, the calculated curve for DPPC had 0.1 unit added to the baseline. The dotted line for PSM was obtained by assuming a stoichiometry of 1:1; its goodness of fit E/N value was 1.17, clearly less good than E/N = 0.41 for a 1.2:1 stoichiometry. The dotted line for POPC tested a stoichiometry of 2:3; its E/N value was 1.84, clearly not as good as the E/N = 0.80 for a 1:1 stoichiometry. The dotted line for DMPS tested a stoichiometry of 1:1; its E/N value was 2.00, not as good as the E/N = 1.44 for a 2:3 stoichiometry.

Article Snippet: The Amplex Red Cholesterol Assay kit was from Life Technologies.

Techniques: Membrane

Journal: Biochemistry

Article Title: STABILITY AND STOICHIOMETRY OF BILAYER PHOSPHOLIPID-CHOLESTEROL COMPLEXES: RELATIONSHIP TO CELLULAR STEROL DISTRIBUTION AND HOMEOSTASIS &

doi: 10.1021/bi400862q

Figure Lengend Snippet: Values for cholesterol-phospholipid complexes *

Article Snippet: The Amplex Red Cholesterol Assay kit was from Life Technologies.

Techniques:

Journal: Biochemistry

Article Title: STABILITY AND STOICHIOMETRY OF BILAYER PHOSPHOLIPID-CHOLESTEROL COMPLEXES: RELATIONSHIP TO CELLULAR STEROL DISTRIBUTION AND HOMEOSTASIS &

doi: 10.1021/bi400862q

Figure Lengend Snippet: Cholesterol distribution coefficients (Q) as a function of cholesterol mole fractions (CMF). Multiple experiments were performed for each curve by incubating 10 mM MBCD bearing 0.02 to 0.10 mM [14C]cholesterol with 0.1–1.0 mM LUV phospholipid for 0.5 h at 25 °C. Solid lines are linear least squares fits. Panel A, POPC (□); DOPC (○); DMPE (▽). Panel B, SOPS (◇); SOPC (▽); POPS (▼). Panel C, DPPC (□); DMPS (■). Panel D, DLPC (△); PSM (●); DMPC, with a linear fit to the portion with a positive slope (○).

Article Snippet: The Amplex Red Cholesterol Assay kit was from Life Technologies.

Techniques:

Journal: Biochemistry

Article Title: STABILITY AND STOICHIOMETRY OF BILAYER PHOSPHOLIPID-CHOLESTEROL COMPLEXES: RELATIONSHIP TO CELLULAR STEROL DISTRIBUTION AND HOMEOSTASIS &

doi: 10.1021/bi400862q

Figure Lengend Snippet: Cholesterol distribution coefficients (Q) related to their thermal transition temperature, Tm. All of the cholesterol distribution coefficients (Q) were obtained using these mixtures: 10 mM MBCD, 0.8 mM LUV phospholipid and 0.1 mM cholesterol. Incubations were for 0.5 h at 25 °C (●) (n = 3–4) and 37 °C (○) (n = 2–3). Tm values were obtained from textbooks and, mostly, the Avanti Polar Lipids catalog. From left to right, the phospholipids are: DOPC, SOPS, POPC, DLPC, SOPC, POPS, DMPC, DMPS, DPPC and PSM. See the Q values in Table 1.

Article Snippet: The Amplex Red Cholesterol Assay kit was from Life Technologies.

Techniques:

Journal: Biochemistry

Article Title: STABILITY AND STOICHIOMETRY OF BILAYER PHOSPHOLIPID-CHOLESTEROL COMPLEXES: RELATIONSHIP TO CELLULAR STEROL DISTRIBUTION AND HOMEOSTASIS &

doi: 10.1021/bi400862q

Figure Lengend Snippet: Correlations of four measures of the strength of association between cholesterol and phospholipids. The four bars give the relative rankings from Table 1 for the respective values for the Kc, the RA of uncomplexed cholesterol, the % free cholesterol at the equivalence point, and Q. Rank 1 denotes the strongest association for a parameter. Values are not given for the Kc of the three phospholipids with stoichiometries of 1:2 and 2:3.

Article Snippet: The Amplex Red Cholesterol Assay kit was from Life Technologies.

Techniques: